Dr. Mohamed Abdelrazek Abdelaleem - د. محمد عبد الرازق عبد العليم

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Dr. Mohamed Abdelrazek Abdelaleem - د. محمد عبد الرازق عبد العليم Scientific page of Dr. Mohamed Abdelrazek Abdelaleem
الصفحة العلمية للدكتور / محمد عبد الرازق عبد العليم

Mohamed Abdelrazek Abdelaleem (Associate Professor - Egyptian Atomic Energy Authority)...

Education:
• Philosophy Doctor (Ph.D.) in Essential Oils Chemistry (Production, Utilization, Application and Therapeutic Effect) from Faculty of Agriculture, Ain Shams University, Egypt (2013), Thesis title “Studies on the Synergistic Effect of some Irradiated Essential Oil in some Food Products”.
• Master

of Science (M.Sc.) in Essential Oils Chemistry (Production, Utilization, Application and Therapeutic Effect) from Faculty of Agriculture, Cairo University, Egypt (2008), thesis title “Effect of Gamma Irradiation on the Biochemical and Microbiological Properties of the Volatile Constituents of Coriander Fruits”.
• Bachelor of Agricultural Sciences (B.Sc.) from Food Science and Technology Department, Factuality of Agriculture, Ain Shams University, Egypt (1998). Attended Human Resource Training Programs:
• Mini MBA in Human Recourse from People Development-EICG (Pre-approved from HRCI).
• Determining Goals Strategy from Canadian Training Center of Human Development (CTCHD) under supervision of Dr. Ibrahim Elfiky.
• Memory Power from Canadian Training Center of Human Development (CTCHD) under supervision of Dr. Ibrahim Elfiky, April 2009.
• Manage Your Time and Stat your Success from Canadian Training Center of Human Development (CTCHD) under supervision of Mr. Ahmed Koddous Allah.
• Certified Professional Leader from Canadian Training Center of Human Development (CTCHD) under supervision of Dr. Ibrahim Elfiky.
• Time Management from People Development-EICG Training Centre.
• Team Building from People Development-EICG Training Centre.
• Effective Business Writing from People Development-EICG Training Centre. Attended Conferences and Training Programs:
• Egyptian Atomic Energy Authority Special Program in “Peaceful Applications of Atomic Energy and Radioisotope Applications” Cairo, Egypt, April 2001.
• Regional (AFRA) Training Course on “Radiation Disinfection and Decontamination of Natural Materials” organized by International Atomic Energy Agency (IAEA) and Egyptian Atomic Energy Authority (EAEA) Held in Cairo, Egypt, April 2003.
• Arab Atomic Energy Agency Training course on “Detection Methods of Irradiated/treated Foods” organized by Arab Atomic Energy Agency (AAEA) and Egyptian Atomic Energy Authority (EAEA) Held in Cairo, Egypt, October 2003.
• Middle Eastern Radioisotope Centre for the Arab Countries Training Course on “Utilization of Radioisotope in Determining the Pesticides and Fertilized Residue in Environment” Held in Cairo, Egypt, November 2007.
• Egyptian Atomic Energy Authority (EAEA) Program in “How to Use International Nuclear Information System (INIS)” Cairo, Egypt, January 2008.
• The 3rd International Conference on Food Science & Nutrition Under the Theme “Nutrition, Nutritional Status & Food Science in Arab Countries” Held in National Research Centre, Cairo, Egypt, November 2008. (The Paper and Poster Awarded " THE BEST POSTER AWARD" in the Conference).
• Egyptian Atomic Energy Authority (EAEA) Program in “Chromatographic Analysis; Principles, Types and Applications” Cairo, Egypt, January 2010.
• Heliopolis Academy Training Center in cooperation with Research Institute of Organic Agriculture in Switzerland (FIBL) Course in "Organic Food Processing", SEKEM Company, Cairo, Egypt, March 2010.
• Participating in a Poster Award in Faculty of Agriculture, Cairo University, Cairo, Egypt, June 2010. Medical Courses:
• "Practical Application of Medical Laboratory Investigations" from Ministry of Health, Faiyum Health Sector, Faiyum General Hospital with Co-operation of : Medical Training Department of International Cultural Center "ICC" Cairo, Egypt, July 2011.

15/08/2026

الأخ العزيز الغالي د. محمد فوزى - أستاذ الزيوت المساعد بمركز البحوث الزراعية والخبير الدولي لزيت الزيتون في ضيافة برنامج الجدعان

0.5 McFarland ...... هذه الطريقة من افضل الطرق المعتمدة للتعرف علي الحمل الميكروبي CFU/mL المراد تحضيره و العمل به في طر...
14/08/2026

0.5 McFarland ......
هذه الطريقة من افضل الطرق المعتمدة للتعرف علي الحمل الميكروبي CFU/mL المراد تحضيره و العمل به في طرق التعرف علي النشاط ضد الميكروبي لبعض العقاقير و الزيوت العطرية والمستخلصات .... وهى من الطرق التى تدربت عليها في بداية شغلي في مجال المبكروبيولجى و تحتاج لخبرة

𝟬.𝟱 𝗠𝗰𝗙𝗮𝗿𝗹𝗮𝗻𝗱: 𝗧𝗵𝗲 𝗦𝘁𝗮𝗻𝗱𝗮𝗿𝗱 𝗕𝗲𝗵𝗶𝗻𝗱 𝗥𝗲𝗹𝗶𝗮𝗯𝗹𝗲 𝗔𝗦𝗧

The 0.5 McFarland standard is a turbidity reference used to standardize the inoculum density of bacterial suspensions before antimicrobial susceptibility testing (AST). It corresponds approximately to 𝟭.𝟱 × 𝟭𝟬⁸ 𝗖𝗙𝗨/𝗺𝗟 for E. coli, but this is only an approximate correlation and varies with bacterial species, size, and shape.

𝗣𝗿𝗶𝗻𝗰𝗶𝗽𝗹𝗲
Traditional McFarland standards are prepared by reacting barium chloride (BaCl₂) with sulfuric acid (H₂SO₄) to produce a fine barium sulfate (BaSO₄) precipitate. The resulting turbidity provides a visual reference for adjusting bacterial suspensions. Commercial standards may use stable latex or other particulate materials.

𝗧𝗿𝗮𝗱𝗶𝘁𝗶𝗼𝗻𝗮𝗹 𝟬.𝟱 𝗠𝗰𝗙𝗮𝗿𝗹𝗮𝗻𝗱 𝗽𝗿𝗲𝗽𝗮𝗿𝗮𝘁𝗶𝗼𝗻
⁃ 50 µL (0.05 mL) of 1.175% BaCl₂·2H₂O
⁃ 9.95 mL of 1% H₂SO₄
⁃ Mix thoroughly to obtain a uniform BaSO₄ suspension.
⁃ Dispense into sealed tubes identical in size and diameter to those used for bacterial suspensions.

𝗖𝗼𝗺𝗺𝗼𝗻 𝗠𝗰𝗙𝗮𝗿𝗹𝗮𝗻𝗱 𝘀𝘁𝗮𝗻𝗱𝗮𝗿𝗱𝘀
0.5 MFU ≈ 1.5 × 10⁸ CFU/mL
1.0 MFU ≈ 3.0 × 10⁸ CFU/mL
2.0 MFU ≈ 6.0 × 10⁸ CFU/mL
3.0 MFU ≈ 9.0 × 10⁸ CFU/mL
4.0 MFU ≈ 1.2 × 10⁹ CFU/mL

𝗛𝗼𝘄 𝗶𝘀 𝗶𝘁 𝘂𝘀𝗲𝗱 𝗶𝗻 𝗔𝗦𝗧?
⁃ Select colonies from a fresh, pure culture.
⁃ Suspend the colonies in sterile saline or suitable broth.
⁃ Mix thoroughly and adjust the turbidity to 0.5 McFarland by visual comparison or, preferably, using a calibrated densitometer.
⁃ The adjusted suspension is used promptly for inoculation; it is not incubated before AST.
⁃ For disk-diffusion testing, inoculate Mueller–Hinton agar (MHA) uniformly to produce a confluent lawn.
⁃ Apply antimicrobial disks and incubate the inoculated plate according to the applicable CLSI or EUCAST method and organism-specific requirements.

𝗜𝗺𝗽𝗼𝗿𝘁𝗮𝗻𝘁 𝗽𝗼𝗶𝗻𝘁𝘀
⁃ 0.5 McFarland ≈ 1.5 × 10⁸ CFU/mL is an approximation, not an exact bacterial count.
⁃ The relationship between turbidity and CFU varies among organisms.
⁃ Vortex/mix both the McFarland standard and test suspension before comparison.
⁃ For visual adjustment, use identical tube dimensions and appropriate lighting.
⁃ A densitometer/turbidimeter provides more objective and reproducible standardization.
⁃ Keep prepared standards tightly sealed and protected from evaporation and contamination.
⁃ BaSO₄ particles may settle or aggregate during storage; mix thoroughly before use and replace standards according to the laboratory's validated SOP/manufacturer's instructions.

Thanks for being a top engager and making it on to my weekly engagement list! 🎉 Salma S Ahmed, Ammar Mohamed, Hamed Aly,...
14/08/2026

Thanks for being a top engager and making it on to my weekly engagement list! 🎉 Salma S Ahmed, Ammar Mohamed, Hamed Aly, Dina Ragab, Sherif Talt, Amr Khaled

08/08/2026
تدريب طلاب الجامعات المصرية علي أساسيات ومبادئ طرق التحليل الكروماتوجرافي المختلفة ....
07/08/2026

تدريب طلاب الجامعات المصرية علي أساسيات ومبادئ طرق التحليل الكروماتوجرافي المختلفة ....

اليوم الاول من تدريب طلاب الجامعات المصرية (عين شمس - القاهرة - حلوان -  الأزهر - بنها - الزقازيق - الفيوم - بني سويف - ...
07/08/2026

اليوم الاول من تدريب طلاب الجامعات المصرية (عين شمس - القاهرة - حلوان - الأزهر - بنها - الزقازيق - الفيوم - بني سويف - الجامعة المصرية الروسية) من جميع التخصصات علي مهارات التنمية البشرية ومهارات التواصل والعرض الفعال

07/08/2026
عندما شعرت بالزلزال منذ لحظات فجر اليوم الإثنين ٣ أغسطس ٢٠٢٦ ..... شعرت بل أيقنت جبروت الله ولطفه وكيف هى الدنيا هشه لا ...
03/08/2026

عندما شعرت بالزلزال منذ لحظات فجر اليوم الإثنين ٣ أغسطس ٢٠٢٦ ..... شعرت بل أيقنت جبروت الله ولطفه وكيف هى الدنيا هشه لا قيمة لها وكيف فزع الناس من مجرد هزة أرضية، فما بالنا بيوم الفزع الأكبر !!!!!!! فهل أعددنا له ...... فتذكرت قول الله عز وجل بسم الله الرحمن الرحيم
إِنَّ إِلَىٰ رَبِّكَ الرُّجْعَىٰ (٨) العلق

03/08/2026

ويبقي السؤال ؟!!!!
كيف حدث تنبيه بوقوع الزلزال قبل حدوثه !!!!!!
علميا يصعب ذلك !!!!

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