06/20/2026
Week 5:
Hey everyone! This week we collected samples from our agricultural sites, and I got to work on my bird extraction from the mist nets*. I worked mainly with extracting House Sparrows (Passer domesticus), but I also included a fun picture of me releasing a Common Yellowthroat (Geothlypis trichas)! I will begin helping to process birds in the coming weeks! It was also a large lab week for me. Batch 1 (the samples we cultured last week) showed no Salmonella growth. However, this week we ran Batches 2 and 3, and we have some Salmonella growth (the black colonies on the red agar)! We are going to run qPCR to confirm that these cultures are, in fact, Salmonella in the coming weeks as well. We also began work on antimicrobial resistance testing using the disk diffusion method. In order to do this, I created a lawn of E. coli on a Mueller-Hinton agar plate with the sample at a 0.5 concentration (determined using a McFarland standard). Then, the plate is sectioned into 4 roughly equal parts, and a disk of medication is centered in each section. The zone of inhibition (the diameter of the ring of no bacterial growth around the medication) is measured to determine if the bacteria is resistant or susceptible to the bacteria. So far, all our samples have tested susceptible to our four antibiotics (Ceftriaxone, Ciprofloxacin, Sulfamethoxazole-trimethoprim, and Tetracycline). Next week, I will be testing Batches 4 and 5 for Salmonella, so stay tuned!
*All birds captured and handled under federal research permits