09/08/2026
Our second-year students were back in the lab this week at the Center for Forensic Science Research and Education for their Forensic Toxicology course! Their first laboratory exercise focused on preparing Neat Calibration Curves and Controls for Co***ne and Fentanyl.
A calibration curve is built from a series of samples with known amounts of the analyte being tested. Once the curve is established, its accuracy is checked using controls, which are separate samples with known analyte concentrations that are run and quantified as if they were unknowns. If the calculated concentration falls within the acceptable range of the method, the curve is considered valid for the quantification of analyte concentrations from unknown biological matrix samples.
Our students calculated the target concentrations for these controls before their preparation. Students then spiked the standard at varying concentrations, added internal standard, dried down, and reconstituted them before the samples were ultimately run on GC/MS and tandem LC/MS instruments.
We love seeing our students back in the lab, happy first week of fall semester classes!