06/02/2026
DPH faculty Dr. Sinan Sousan and Dr. David Tulis [Department of Physiology], serving together as Principal Investigators, along with Co-Investigator Dr. Nathan Hudson [Department of Physics], recently received a $70,921 pilot project grant from the Center for Human Health and the Environment (CHHE) for their project titled "GPR68: a promising new diagnostic tool to control vascular pathology from E-cig secondhand exposure". Based at NC State University, the CHHE Pilot Project Program fosters interdisciplinary collaborations and provides support for research aimed at understanding the adverse impacts of environmental factors on human health and disease.
This interdisciplinary project brings together two distinct fields of study to tackle the cardiovascular impacts of secondhand e-cigarette aerosols.
The research is driven by a novel biological concept originating from Dr. Tulis's laboratory. Dr. Tulis has extensive expertise characterizing the molecular and cellular mechanisms underlying abnormal blood vessel growth in cardiovascular disease. His lab recently made exciting discoveries regarding the acid-sensing receptor GPR68 and its role in inhibiting vascular smooth muscle (VSM) cell proliferation.
By merging Dr. Tulis's innovative cellular models with Dr. Sousan's previous research, which quantified extreme secondhand fine particulate matter (PM2.5) exposure from e-cigarettes, the team can now test this pathway holistically. Using wild-type and knockout mice alongside human coronary cells, the study will analyze how different e-liquid aerosol mixtures activate the GPR68 receptor to identify new biomarkers and therapies for cardiovascular risks. Dr. Hudson will direct the quantitative confocal analysis of these cellular signals to evaluate biological sensing capacity.
Congratulations to the team on this exciting collaboration, which beautifully bridges environmental exposure science with cutting-edge cardiovascular physiology!