11/16/2021
The Biotechnology Center, Flow Cytometry has a new name: Cytometry & Microscopy to Omics (CMtO).
While maintaining our long-term core services in cytometry, the CMtO core will also utilize advanced microscopy techniques to gain a deeper understanding of cell sorting and analysis, while also enabling cutting-edge spatial and single cell omics. The facility is therefore being instrumented to integrate cytometry and microscopy with omics to offer 10x Genomics Spatial Gene Expression and 10X Chromium Single Cell Gene Expression, as well as Proteomics on the same tissue sections and single cells. As one of the instruments required to accomplish these goals, the CMtO facility has recently purchased a Thermo Fisher Bigfoot Spectral Cell Sorter and is evaluating investments in next generation cytometry as a means to update and enhance its current analyzers across the campus. To facilitate this transition, the CMtO facility is also acquiring a group of support instruments, which include a BSL2 capable Cryostat, Microtome, Thermal Cyclers, and Cell Counters. In addition, the Zeiss LSM 980 with Airyscan 2 is being constructed as an integrated confocal and widefield microscope that will include Spectral-GaAsP, dedicated NIR Lasers and detectors, 2-photon capabilities, and custom configured color and black and white cameras. This robust state-of-the art fundamental instrumentation platform will fully support current and future Visium Transcriptomics and Proteomics projects. Please contact Dr. Sivaguru, Director of CMtO ([email protected]) for further information about these exciting and game changing developments for the services offered.
CMtO Webpage: https://biotech.illinois.edu/cmto
New Email for Appointments, Training and Project Consultation: [email protected]